http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/128 Annual Research sessions held in the year 2018 Sun, 05 Apr 2026 16:46:19 GMT 2026-04-05T16:46:19Z http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/321 8th ANNUAL RESEARCH SESSION SABARAGAMUWA UNIVERSITY OF SRI LANKA Wed, 19 Dec 2018 00:00:00 GMT http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/321 2018-12-19T00:00:00Z http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/319 Effect of Vascular Endothelial Growth Factor 165b and (VEGF165b) KIT-ligand/Stem Cell Factor (SCF) on Porcine Primordial Follicle Viability Invitro Madushanka, D.N.N; Magamage, , Manjula P.S.; Mayurawansha, W.R.A.S Vascular endothelial growth factor (VEGF) is well known for its ability to regulate angiogenesis where it has been reported of playing a crucial role in metastasis of tumors. Stem cell factor (SCF) plays a crucial role in cell viability. Growth of a mammalian oocyte starts with an avascular structure called primordial follicle’ and after the ovulation this avascular structure transforms into a heavily vascular corpus luteum. Primordial follicle activation has been regulated by many intrinsic and extrinsic factors of animals many of which have not been studied completely. It has been observed that the VEGF and its isomers have regulatory effects on the formation of the vascular bed in developing follicles and it supports the activation of the dormant follicles. The objective of the current study was to determine the effect of anti-angiogenic VEGF165b and SCF on porcine primordial follicle development in vitro. VEGF165b is known to be an anti-angiogenic factor where SCF is well known for its ability to enhance cell migration, proliferation and cell survival. The tissue samples were treated with VEGF165b with 0ng/ml, 0.1, 1.0, 10.0 ng/ml and day 0 (Negative control) samples were fixed instantly in 10% neutral buffered formalin and each treatment had 6 replicates. Separate three trials were conducted in order to observe the rest of the effects in each treatment. All the treatments were supplemented with 10ng/ml of SCF to stabilize the cell survival. All the treated tissues were subjected to 72hrs of incubation under 5% CO2 with the humidified atmospheric conditions at 37.50C and followed by a histological assay to obtain the preliminary data. Out of three different dose regimes in VEGF165b treated tissues, 0.1ng/ml has shown 65.15% of follicle viability. It was numerically the highest recorded viable follicle count whereas 1.0ng/ml and 10.0ng/ml treatments reported 39.61% and 20.20% follicle viability respectively. Although, 0.1ng/ml VEGF165b showed the highest viable follicle count, it was lower than the viability showed in previous study with 0.1ng/ml VEGF165a (92.6%). It was proposed under natural conditions these VEGF isomers and SCF were in an equilibrium which regulated the angiogenesis and anti-angiogenesis. In conclusion it was evident that VEGF165b did not support the follicle viability alone, even with the presence of SCF that stabilized the cell viability. Further studies are necessary for understanding the exact role of VEGF165b and SCF combination effect on cellular viability which may bring new insights into cancer therapy Wed, 19 Dec 2018 00:00:00 GMT http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/319 2018-12-19T00:00:00Z http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/316 Immunomodulatory Fungal Polysaccharides: Prospective Candidates for the Development of New Nutraceuticals and Adjuvants Perera, Namal; Yang, Fang-Ling; Huad, Kuo-Feng; Wuc, Shih-Hsiung Naturally occurring complex carbohydrates represent a structurally diverse group of macromolecules which shows a broad range of biological activities. However, their clinical contribution is still limited because of the structural complexity, lack of studies on structure activity relationship and underlying mechanisms of biological activity of individual polysaccharide components. In the present study polysaccharides were isolated from the cold-water extracts of two mushroom species, Antrodia cinnamomea and Auricularia auricula-juadae, to investigate their immunostimulatory potential and mechanisms of action. Polysaccharides were purified by size exclusion and ion exchange chromatography and their chemical structures were elucidated by Gas Chromatography-Mass Spectroscopy (GC-MS) and Nuclear Magnetic Resonance Spectroscopy (NMR). Immunostimulatory properties of each polysaccharide were investigated targeting the proinflammatory cytokines (TNF-, IL-6) and nitric oxide (NO) producing abilities in selected immune cell models (J774.1A and Raw 264.7). Immunologically active polysaccharide of A. cinnamomea (ACP; MW>70 kDa) was chemically identified as galactomannan, with branched octasaccharide repeating units, whereas polysaccharide of A. auricula-ju dae (AAPS, MW>70 kDa) was identified as glucuronoxylomannan with a heptasaccharide repeating units. O-acetyl modification at mannose and xylose residues was observed in AAPS while glucuronic acid residues remained unacetylated. Both ACP and native AAPS activated murine macrophage cells to produce TNF- and IL-6 in a concentration dependent manner. Toll like receptor 4 was identified as the main receptor involved in ACP and AAPS mediated macrophage activation. Complete abrogation of immunostimulatory properties was observed in deacetylated and carboxyl reduced AAPS indicating the essentiality of both acetyl and carboxylic functionalities in immunostimulatory process. Molecular modelling data further demonstrated the role of acetyl and caroboxyl moieties in receptor binding. Our findings have provided firm scientific evidences for the immunoenhancing properties of two mushroom species and the potential of these two polysaccharides to be strong candidates for the development of new carbohydrate-based nutraceutical supplements and adjuvants in the treatment of immunity related disorders in near future. Wed, 19 Dec 2018 00:00:00 GMT http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/316 2018-12-19T00:00:00Z http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/312 Resource Management Approaches Based on Nature Inspired Algorithms in Cloud Computing Jayasena, K.P.N; Lib, Lin Cloud computing is an emerging computing platform with flexible computational architecture and an enormous collection of heterogeneous systems. Resource management is an important strategy in enhancing the overall performance of cloud computing. Currently, effective resource management in cloud computing is becoming an inevitable demanding topic and it is very challenging to implement efficient resource management based on QoS demands by optimizing the number of objectives. In this research, resource management problem can be considered from three optimization aspects: single objective optimization, multi-objective optimization and many objective optimizations. We discuss two significant levels in this emerging resource management paradigm: one is task scheduling, that maps tasks to VMs and the other is VM placement, which maps VMs to physical servers. The nature inspired algorithm approaches can obtain feasible solutions than others. Firstly, this research explores the research in the area of resource management in cloud computing to gain an understanding of related work. Secondly, this research proposes single-objective efficient resource management and task scheduling methods in cloud platforms to minimize response time, makespan and data center processing time. Another main target of the thesis is to research multi-objective energy aware, efficiency aware and QoS aware resource management as VM placement mechanism for simultaneously optimizing three objectives. Most of the previous research methods, either single objective or multi-objective, cannot provide suitable solutions when the number of the objectives exceeds three. Therefore, there is a tremendous demand to use the many-objective optimization mechanism to deal with the resource-management problem. Finally, this research proposes a many-objective approach for VM placement to attain equilibrium among five objectives. In summary, we propose energy aware, efficiency aware, cost aware, QoS aware load balancing and utilization aware resource scheduling approaches in cloud computing using nature-inspired algorithms. The performance evaluation from three optimization aspects, i.e., single-objective, multi-objective and manyobjective optimizations, demonstrate that the proposed approaches are capable of enhancing the state-of-the-art techniques in the environment of enormous cloud data centers by optimizing different objective functions. Wed, 19 Dec 2018 00:00:00 GMT http://repo.lib.sab.ac.lk:8080/xmlui/handle/123456789/312 2018-12-19T00:00:00Z