Abstract:
Plants are the one of the glorious and fascinating source of natural biopesticides. The present
investigation was carried out for pytochemical analysis and to assess the nematicidal and
nematostatic potential of aqueous extract of some plant parts viz., Ageratum conyzoides and
Coccinia grandis against hatching and nematicidal behavior of root-knot nematode, Meloidogyne
incognita in vitro conditions. The second stage juveniles (J2) were exposed at 24, 48 and 72 hours
in diff erent concentration (S, S/2, S/10, and S/100) of plant extracts. During in vitro condition
inhibition of egg hatchability and J2
juveniles’ mortality varied according to the concentration
of plant extract. The extract of Ageratum conyzoides leaves and stem exhibited highly promising
mortality (98-100%) after 72 hours of exposure periods while the plant extract of C. grandis
leaves and fruit showed minimum mortality (56-60%) after 24 hours of exposure periods. There
was a gradual decrease in egghatching with an increase in the concentration of aqueous extract
of plants. A. conyzoides leaves and stem extract elucidating most eff ectiveness in reducing
egghatching and increase in mortality of J2
juveniles of M. incognita. Concentration of the extract
was directly proportional to the mortality of second stage juveniles and inversely proportional to
the egghatching. Phytochemical analysis in various solvent of diff erent polarity such as ethanol,
methanol, acetone, chloroform, petroleum and water marked the impressive outcome of alkaloids,
saponins, tannins, fl avonoids, protein, amines, glycosides, carbohydrate, steroids, mucilage, gums,
terpenoids and phlobatannins in the plant extracts. The aqueous extract of Ageratum conyzoides
showed maximum egg inhibition and J2
juvenile mortality due to the presence of phytoconstituents
such as alkaloid, tannins, phenol, saponins, glycosides, fl avonoids, carbohydrate, protein,
mucilage/gum and phlobatannins while Coccinia grandis showed minimum egg inhibition and
juvenile mortality.
