Bioremediation of Dairy Waste Water using Lipid Degrading Microbial Isolates

Abstract

Human establishment on earth and the technical advancement followed by, has created water as t resource in the world. Waste water has brought severe environmental and social impacts. Study is an investigate the water pollution caused by lipids in the domestic and industrial sewage which influenced flora and fauna and to come u p with appropriate remedies. The immiscible nature of lipids with wat difficult to be degraded and removed via conventional treatment systems. The interest towards lipid water treatment by microbial isolates has been rejuvenated due t o the high operational and mainte associated with conventional treatment systems. Study was attempted to isolate microorganisms capable of degrading lipids efficiently from diverse e Hundred strains were screened and six strains were lipolytic positive. Among the lipolytic positive strains, three strains (S1,S3 and S5) exhibited the cell growth OD600=1 after 48 hours of cultivation. The optimum temperature was found to be 30 ºC for maximum during 48 hours of culturing. Subsequently the lipid degradation competency of each strain was investig 30 ºC for about 24 hours where, the S1 yielded the highest lipid degradation efficiency (39.02±0.01% ). S1 was accustomed for investigating the lipase activity (2.605±0.01) and the lipid degradation (39.02±0.01%) against cell growth. S1, S3 and S5 were identified in accordance with morphologic biochemical testing and 16S rRNA sequencing as Pseudomonas aeruginosa, Enterobacter cl Acinetobacter pittii respectively. water drainage columns. Clogging of drainpipes, corrosion of sewer pipes resulted due to the accum FOGs and environmental pollution occurred creating unpleasant odor. Therefore, it is all-important to act on proper treatments to remove lipids from wastewater. physicochemical waste water treatment methods that have been studied so far are sedimentation degasification, screening, aeration, ozonation, coagulation, neutralization, chlorination, ion exchange an Certain boundaries such as high cost, partial separation, occurrence of secondary pollutants and solid large quantities and usage of chemicals associated with physicochemical strategies have emplaced approaches as the promising candidates towards lipid contained waste water treatment.