Abstract:
Mammalian folliculogenesis is a complex process which determines the availability of
viable oocyte for successful fertilization. Initiation of mammalian primordial follicle
development also known as primordial follicle activation is poorly understood. Vascular
endothelial growth factor 165a (VEGFA165a) is a well-known angiogenic factor which
stimulate the vascular bed formation in cows with cystic ovarian lesions. Therefore,
studies on VEGFA role on ovarian follicle activation are indispensable. The objective of the
current study was to determine the different dose regime effect of VEGF165a on porcine
primordial follicle development in vitro. This preliminary data was obtained from shortterm (72 hours) in vitro culture of porcine ovarian cortical stripes under 5% CO2 and 95%
O2 with the humidified atmospheric conditions. Out of three different dose regimes,
0.1ng/ml (10-6M) has shown highest viable follicles numbers (22.40±0.16) compared to
1ng/ml (10-5 M) (22.36±0.15) and 10ng/ml (10-4M) (7.0±0.0). Dose regime 10ng/ml
VEGFA165a treatment has shown the accelerated follicle degeneration (20.11±0.11). For
the first time, here we reported that lower concentration of VEGFA165a provide more
follicle viability in porcine tissues under short term culture conditions. All the data were
analyzed using SAS 9.0 and the treatment effect was not significant among any treatment
nor with the control (p>0.05). In conclusion, it was evident in this study that the lowest
concentrations of VEGFA165a has increased the follicle viability while higher
concentrations implicit increased follicle degeneration. Acknowledgements: This study
was supported by National Science Foundation of Sri Lanka grant NSF/SCH/2015/07 and
Sabaragamuwa University Research Grant, SUSL/RG/2015/06.
