Application of Seed Treatments to Increase Germinability of Cardamom (Elettaria cardamomum) Seeds under in vitro Conditions

Abstract

Cardamom (Elettaria cardamomum) has great commercial value as a spice in Sri Lanka. Due to the presence of hard seed coat, cardamom has low seed germination potential. Therefore the present study was conducted to study the potential to improve the seed germination by applying different mechanical, physical and chemical treatments and to develop a cost effective and rapid seed germination method in in vitro conditions. Complete Randomized Design (CRD) with ten replicates was used for the study. The seeds of cardamom were treated with 40 different seed treatments and surface sterilized by using 20% clorex. MS basal medium was used without plant growth regulators and solidified by 0.6% agar. Anova (DMRT) test showed that there were significant effects at p<0.05 level on seed germination of cardamom seeds with different seed treatments. According to the results 50% HNO3 for 15 min exposure was the best treatment to obtain higher number of germinated seeds (90%) within 6 weeks upto 0.6 cm height. The second and third best treatments were 75% HNO3 for 15 min and 75% HNO3 for 10 min which showed 80% and 65% seed germination percentages respectively. This experiment showed chemical treatment (HNO3 ) is the best to induce seed germination of E. cardamomum in in vitro conditions. The results obtained in this study will be important for plant breeders and farmers who cultivate these species commercially.