Abstract:
Enterobacter sakazakii is a gram-negative rod within the family Enterobacteriaceae,
genus Enterobacter. This is a frequent contaminant in powdered formula and can
cause sepsis meningitis or necrotizing enterocolitis in newborn infants. Therefore it is
very important to implement an effective method to identification of Enterobacter
sakazakii in powdered milk.
Three methods were carried out in order to determine the most effective pathway to
identify Enterobacter sakazakii by combining modem methods that use to detect
Enterobacter sakazakii In first method samples were tested with Violet Red Bile
Glucose Agar and dark purple colony was sub cultured in Nutrient Agar and yellow
colonies formed were confirmed with oc-glucosidase and API 20E kit. Second
procedure followed the Lauryl sulfate tryptose broth with extra salt and vancomycin
cultivation and sub cultured in Nutrient agar + bile salts broth, oc-glucosidase and API
20E kit was used for confirmation. In the 3 procedure sample was mixed aseptically
with BPW (Buffered peptone water) and incubated. 0.1ml of solution was transferred
in to LSD + SV solution and the same method in procedure 2 was followed to identify
Enterobacter sakazakii
The results obtained from three procedures revealed that no Enterobacter sakazakii
was present in food samples taken for assessment. No results were found to reveal
that is present in the environmental samples but results shown the presence of many
Enterobacteriaceae species which are not harmful.
The second procedure is to be the rapid sample compared to other two methods and
most effective method in identification is one that can be recommended that the
procedure one is the most cost effective method in routing analysis of Enterobacter
sakazakii.
