CHEMISTRY AND BIOACTIVITY OF STEM BARK OF Artocarpus nobilis

Abstract

The dried, ground stem bark of Artocarpus nobilis was extracted with cold methanol. The extract was evaporated to dryness under reduced pressure using rotary evaporator. The methanol extract was partitioned with nbutanol and water. The n-butanol crude extract was subjected to antifungal activity test against Cladosporium cladosporioides by TLC bio-autography method The n-butanol extract was shown positive response; at the meantime the above n-butanol extract was indicating that absence of alkaloids. In order to isolate two antifungal active compounds and an inactive compound, the n-butanol extract was subjected to combination of chromatography such as normal silica gel, reversed phase Ci8 silica,Sephadex LH-20, and HPLC. The final purification of two antifungal active compounds and an inactive compound was achieved by high performance liquid chromatography (HPLC). Their NMR, IR, Mass, UV, Physical constants, and structure determination studies are also in progress.