Abstract:
Environmental remediation has been used to solve environmental pollution problems
effectively and efficiently. Microorganisms can be used in bioremediation processes to
remove residual pesticides from the ecosystems. Pesticides have been widely used in
agriculture to produce high-yielding, genetically improved crops, in order to achieve the
zero hunger goal. However, pesticides indirectly pollute the natural water bodies due to
the presence of pesticide residues, and such contaminated water has resulted in acute
and chronic health effects. Therefore, this study aimed to find pesticide bioremediation
bacteria that are regionally appropriate. In the study, 23 bacterial isolates (isolate 01
to isolate 23) were isolated from collected surface water samples, after being enriched
for five days. The tolerance capacity of profenofos was then examined in all bacterial
isolates resulting with optimal levels for four isolates (isolate 01, 03, 13, and 18), which
were chosen for further investigation. Their pesticide degradation capability at 5-day
intervals was determined using GC-MS, while the pesticide resistivity was determined
using, a plate count. The results showed that the tolerance capacity of all isolates are
not the same. According to the ANOVA followed by the Post Hoc Test, bacterial isolate
03 had a significantly high ability to break down profenofos (45.40% rate within the
first 10 days of the study), while isolates 01, 03, 13, and 18 were able to withstand
pesticides for 20 days in M9 minimal salt medium (MSM), isolates 01, 13, and 18 were
unable to display a deterioration pattern. According to the results, isolate 03 is capable
of decomposing profenofos at a high rate, while other isolates are only resistant to the
pesticide.
