Abstract:
Biopolymer-based active edible films are regarded as potential alternatives to plastic packaging
because of their superior biodegradability, edibility, sustainability, and biocompatibility. In this
study, active edible films from alginate (Alg) containing various concentrations (0.5%, 1%, and
1.5%) of active ingredients such as ascorbic acid (AA) and lemongrass essential oil (LEO)
were developed using CaCl2 as the crosslinking agent using the casting method. The physical,
optical, mechanical, thermal, antioxidant, and antimicrobial properties of the fabricated films
were investigated to determine the potential of using them in food applications. The results
revealed that the addition of AA and LEO with increasing concentration has increased the film
thickness and decreased the moisture content, which ranged from 0.14 mm to 0.30 mm and 26.8%
to 10.5%, respectively. Significantly enhanced water resistance was recorded for alginate-based
films containing LEO (Alg-LEO) compared to alginate-based films containing AA (Alg-AA) due
to the hydrophobic nature of LEO. Although there was a significant decrease (p < 0.05) in tensile
strength with the addition of AA and LEO, elongation at break was significantly increased
(p < 0.05) when increasing LEO concentration. Both films showed a total color difference
(△E), which significantly increased (p < 0.05) as a function of antioxidant concentration. The
thermogravimetric analysis results showed that LEO was more efficient at improving the thermal
stability of the films compared to AA. The incorporation of 1% AA and 1.5% LEO were the
most effective concentrations in controlling the growth of Staphylococcus aureus, Bacillus cereus,
Klebsiella pneumoniae, and Escherichia coli, while Bacillus cereus being the most susceptible
bacteria. The 1.5% Alg-AA films showed stronger DPPH radical scavenging activities (IC50 =
50.4 µg/mL) than the 1.5% Alg-LEO films (IC50 = 76.9 µg/mL). These results suggest that the
developed alginate-based films containing AA and LEO can be used as sustainable and active
materials in the food packaging industry.
